Article
Attenuating mutations in glycoproteins E1 and E2 of Sindbis virus produce a highly attenuated strain when combined in vitro.
Journal of virology - 1 Sept 1990
Polo J M, Johnston R E
Abstract excerpt
Alterations in either the E1 or the E2 glycoprotein of Sindbis virus can affect pathogenesis in animals. Previously, we identified two distinct E1 glycoprotein gene sequences which differed in their effect on pathogenesis. One had an attenuation phenotype following subcutaneous inoculation of neonatal mice (E1 Ala-72, Gly-75, and Ser-237), while the other was virulent (E1 Val-72, Asp-75, and Ala-237). In this...
Topics
- Animals
- Animals, Newborn
- Base Sequence
- Cell Line
- DNA, Recombinant
- DNA, Viral
- Genes, Viral
- Kinetics
- Membrane Glycoproteins
- Mice
- Molecular Sequence Data
- Mutation