Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Amino acid changes responsible for attenuation of virus neurovirulence in an infectious cDNA clone of the Oshima strain of Tick-borne encephalitis virus

23 Mar 2004

Abstract excerpt

A stable full-length infectious cDNA clone of the Oshima strain of Tick-borne encephalitis virus (Far-Eastern subtype) was developed by a long high-fidelity RT-PCR and one-step cloning procedure. The infectious clone (O-IC) had four amino acid substitutions and produced smaller plaques when compared with the parent Oshima 5-10 strain. Using site-directed mutagenesis, the substitutions were reverted to restore the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Mosquito-borne diseases and control
Share to Vector-borne infectious diseases
Share to Viral Infections and Vectors

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Source

Identifiers

PMID: 15039543
DOI: 10.1099/vir.0.19668-0

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Amino acid changes responsible for attenuation of virus neurovirulence in an infectious cDNA clone of the Oshima strain of Tick-borne encephalitis virusPMID 15039543Read ↗

The degree of attenuation of tick-borne encephalitis virus depends on the cumulative effects of point mutations.Semantic proximity Read ↗

A molecularly cloned, live-attenuated japanese encephalitis vaccine SA14-14-2 virus: a conserved single amino acid in the ij Hairpin of the Viral E glycoprotein determines neurovirulence in mice.Semantic proximity Read ↗

A BHK-21 cell culture-adapted tick-borne encephalitis virus mutant is attenuated for neuroinvasiveness.Semantic proximity Read ↗

Spontaneous and Engineered Deletions in the 3′ Noncoding Region of Tick-Borne Encephalitis Virus: Construction of Highly Attenuated Mutants of a FlavivirusSemantic proximity Read ↗

A simple method for developing an infectious cDNA clone of Japanese encephalitis virus.Semantic proximity Read ↗

Attenuation of tick-borne encephalitis virus using large-scale random codon re-encoding.Semantic proximity Read ↗

Comparison of the live-attenuated Japanese encephalitis vaccine SA14 -14-2 strain with its pre-attenuated virulent parent SA14 strain: similarities and differences in vitro and in vivoSemantic proximity Read ↗

Characterization of a Highly Attenuated Japanese Encephalitis Virus Generated from Molecularly Cloned cDNASemantic proximity Read ↗

Recovery of a chemically synthesized Japanese encephalitis virus reveals two critical adaptive mutations in NS2B and NS4ASemantic proximity Read ↗

Infectious cDNA Clone of Attenuated Langat Tick-Borne Flavivirus (Strain E5) and a 3′ Deletion Mutant Constructed from It Exhibit Decreased Neuroinvasiveness in Immunodeficient MiceSemantic proximity Read ↗

Genetic Determinants of Japanese Encephalitis Virus Vaccine Strain SA14-14-2 That Govern Attenuation of Virulence in Mice.Semantic proximity Read ↗

Variable region of the 3′ UTR is a critical virulence factor in the Far-Eastern subtype of tick-borne encephalitis virus in a mouse modelSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.