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Fluorescence-activated cell sorting and directed evolution of α-N-acetylgalactosaminidases using a quenched activity-based probe (qABP).

Chemical communications (Cambridge, England) - 21 Aug 2013

Kalidasan Kamaladasan, Su Ying, Wu Xiaoyuan, Yao Shao Q, Uttamchandani Mahesh

Abstract excerpt

An α-N-acetylgalactosamine-containing quenched activity-based probe (qABP) was designed and successfully synthesized, and it was subsequently used in directed enzyme evolution experiments aided by fluorescence-activated cell sorting (FACS) for the discovery of an α-N-acetylgalactosaminidase varia...

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PMID: 23842736
DOI: 10.1039/c3cc42836b

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-23842736-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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