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Article

High-throughput screening methodology for the directed evolution of glycosyltransferases.

Nature methods - 1 Aug 2006

Aharoni Amir, Thieme Karena, Chiu Cecilia P C, Buchini Sabrina, Lairson Luke L, Chen Hongming, Strynadka Natalie C J, Wakarchuk Warren W, Withers Stephen G

Abstract excerpt

Engineering of glycosyltransferases (GTs) with desired substrate specificity for the synthesis of new oligosaccharides holds great potential for the development of the field of glycobiology. However, engineering of GTs by directed evolution methodologies is hampered by the lack of efficient screening systems for sugar-transfer activity. We report here the development of a new fluorescence-based high-throughput...

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Identifiers

PMID: 16862135
DOI: 10.1038/nmeth899

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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