Article
Directed evolution of lectins by an improved error-prone PCR and ribosome display method.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014
Hu Dan, Tateno Hiroaki, Hirabayashi Jun
Abstract excerpt
Lectins are useful reagents for the structural characterization of glycans. However, currently available lectins have an apparent drawback in their "repertoire," lacking some critical probes, such as those for sulfated glycans. Thus, engineering lectins with novel specificity would be of great practical value. Here, we describe a directed evolution strategy to tailor novel lectins for novel specificity or...
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