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Article

A high-throughput fluorescence-based glycosyltransferase screen and its application in directed evolution.

Nature protocols - 1 Jan 2008

Williams Gavin J, Thorson Jon S

Abstract excerpt

This protocol details the application of a high-throughput fluorescence-based screen, in conjunction with error-prone PCR/saturation mutagenesis, for altering the proficiency and/or promiscuity of a secondary metabolite glycosyltransferase (GT) via directed evolution. Given the structural and mechanistic similarities among secondary metabolite-associated GTs, this approach may provide a template for engineering...

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Identifiers

PMID: 18323806
DOI: 10.1038/nprot.2007.538

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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