Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Fluorescence activated cell sorting as a general ultra-high-throughput screening method for directed evolution of glycosyltransferases.

Journal of the American Chemical Society - 4 Aug 2010

Yang Guangyu, Rich Jamie R, Gilbert Michel, Wakarchuk Warren W, Feng Yan, Withers Stephen G

Abstract excerpt

Glycosyltransferases (GTs) offer very attractive approaches to the synthesis of complex oligosaccharides. However, the limited number of available GTs, together with their instability and strict substrate specificity, have severely hampered the broad application of these enzymes. Previous attempts to broaden the range of substrate scope and to increase the activity of GTs via protein engineering have met with...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Campylobacter jejuni
Share to Escherichia coli

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Flow Cytometry

Share to Gene Expression

Share to Glycosyltransferases

Share to High-Throughput Screening Assays

Share to Mutation

Source

Identifiers

PMID: 20662530
DOI: 10.1021/ja104167y

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Fluorescence activated cell sorting as a general ultra-high-throughput screening method for directed evolution of glycosyltransferases.PMID 20662530Read ↗

High-throughput screening methodology for the directed evolution of glycosyltransferases.Semantic proximity Read ↗

Fluorescence-activated cell sorting and directed evolution of α-N-acetylgalactosaminidases using a quenched activity-based probe (qABP).Semantic proximity Read ↗

A high-throughput fluorescence-based glycosyltransferase screen and its application in directed evolution.Semantic proximity Read ↗

Single-mutations at the galactose-binding site of enzymes GalK, GalU, and LgtC enable the efficient synthesis of UDP-6-azido-6-deoxy-d-galactose and azido-functionalized Gb3 analogs.Semantic proximity Read ↗

A high-throughput pH indicator assay for screening glycosyltransferase saturation mutagenesis libraries.Semantic proximity Read ↗

Directed Evolution of Therapeutic Antibodies Targeting Glycosylation in CancerSemantic proximity Read ↗

Structure-based engineering of E. coli galactokinase as a first step toward in vivo glycorandomization.Semantic proximity Read ↗

Glycosyltransferases as versatile tools to study the biology of glycansSemantic proximity Read ↗

Enhancing the soluble expression of α-1,2-fucosyltransferase in E. coli using high-throughput flow cytometry screening coupled with a split-GFP.Semantic proximity Read ↗

Digital screening methodology for the directed evolution of transglycosidases.Semantic proximity Read ↗

Chemoenzymatic synthesis of unusual antibody glycoforms using E. coli expressed human and bovine β-1,4-galactosyltransferase and their mutants.Semantic proximity Read ↗

Combinatorial genome and protein engineering yields monoclonal antibodies with hypergalactosylation from CHO cellsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.