Structural and thermodynamic insight into Escherichia coli UvrABC-mediated incision of cluster diacetylaminofluorene adducts on the NarI sequence.

Abstract excerpt

Cluster DNA damage refers to two or more lesions in a single turn of the DNA helix. Such clustering may occur with bulky DNA lesions, which may be responsible for their sequence-dependent repair and mutational outcomes. Here we prepared three 16-mer cluster duplexes in which two fluoroacetylaminofluorene adducts (dG-FAAF) are separated by zero, one, and two nucleotides in the Escherichia coli NarI mutational hot...

Topics

• 2-Acetylaminofluorene
• Base Pairing
• Base Sequence
• Cluster Analysis
• DNA Adducts
• DNA Repair
• Deoxyribonucleases, Type II Site-Specific
• Endodeoxyribonucleases
• Escherichia coli
• Escherichia coli Proteins
• Molecular Dynamics Simulation
• Mutation