Article
Exocyclic amino groups of flanking guanines govern sequence-dependent adduct conformations and local structural distortions for minor groove-aligned benzo[a]pyrenyl-guanine lesions in a GG mutation hotspot context.
Nucleic acids research - 1 Jan 2007
Rodríguez Fabián A, Cai Yuqin, Lin Chin, Tang Yijin, Kolbanovskiy Alexander, Amin Shantu, Patel Dinshaw J, Broyde Suse, Geacintov Nicholas E
Abstract excerpt
The environmental carcinogen benzo[a]pyrene (BP) is metabolized to reactive diol epoxides that bind to cellular DNA by predominantly forming N2-guanine adducts (G*). Mutation hotspots for these adducts are frequently found in 5'- ... GG ... dinucleotide sequences, but their origins are poorly understood. Here we used high resolution NMR and molecular dynamics simulations to investigate differences in G* adduct...
Topics
- Amines
- Base Sequence
- Benzopyrenes
- DNA Adducts
- Deoxyguanosine
- Guanine
- Models, Molecular
- Mutation
- Nuclear Magnetic Resonance, Biomolecular
- Nucleic Acid Conformation
- Protons
- Temperature