Article
Rapid apolipoprotein E phenotyping by immunofixation in agarose.
Journal of lipid research - 1 May 1991
Luley C, Baumstark M W, Wieland H
Abstract excerpt
Conventional determination of apolipoprotein E isomorphs comprises ultracentrifugation of 1-5 ml serum, delipidation of very low density lipoproteins (VLDL), and isoelectric focusing (IEF) in polyacrylamide gels. In order to reduce the sample volume and to avoid nonspecific protein bands, immunoblotting was proposed. Now we describe a methodological variant that uses 25 microliters serum, replaces...
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