Rapid apolipoprotein E phenotyping by immunofixation in agarose.

Article

Rapid apolipoprotein E phenotyping by immunofixation in agarose.

Journal of lipid research - 1 May 1991

Luley C, Baumstark M W, Wieland H

Abstract excerpt

Conventional determination of apolipoprotein E isomorphs comprises ultracentrifugation of 1-5 ml serum, delipidation of very low density lipoproteins (VLDL), and isoelectric focusing (IEF) in polyacrylamide gels. In order to reduce the sample volume and to avoid nonspecific protein bands, immunob...

Topics

Apolipoproteins E
Chemical Precipitation
Humans
Immunologic Techniques
Isoelectric Focusing
Phenotype
Sepharose

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