A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases.

Abstract excerpt

Precise and effective genome-editing tools are essential for functional genomics and gene therapy. Targeting nucleases have been successfully used to edit genomes. However, whole-locus or element-specific deletions abolishing transcript expression have not previously been reported. Here, we show heritable targeting of locus-specific deletions in the zebrafish nodal-related genes squint (sqt) and cyclops (cyc)....

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Chromosome Deletion
• Share to Deoxyribonucleases