Article
Molecular signature of high yield (growth) influenza a virus reassortants prepared as candidate vaccine seeds.
PloS one - 1 Jan 2013
Ramanunninair Manojkumar, Le Jianhua, Onodera Shiroh, Fulvini Andrew A, Pokorny Barbara A, Silverman Jeanmarie, Devis Rene, Arroyo Jennifer M, He Yu, Boyne Alex, Bera Jayati, Halpin Rebecca, Hine Erin, Spiro David J, Bucher Doris
Abstract excerpt
BACKGROUND: Human influenza virus isolates generally grow poorly in embryonated chicken eggs. Hence, gene reassortment of influenza A wild type (wt) viruses is performed with a highly egg adapted donor virus, A/Puerto Rico/8/1934 (PR8), to provide the high yield reassortant (HYR) viral 'seeds' for vaccine production. HYR must contain the hemagglutinin (HA) and neuraminidase (NA) genes of wt virus and one to six...
Topics
- Genome, Viral
- Hemagglutinins
- Humans
- Influenza A Virus, H1N1 Subtype
- Influenza A Virus, H3N2 Subtype
- Influenza A Virus, H5N1 Subtype
- Influenza A virus
- Influenza Vaccines
- Mutation
- Neuraminidase