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Article

Analytical FcRn affinity chromatography for functional characterization of monoclonal antibodies.

mAbs - 1 Jan 2000

Schlothauer Tilman, Rueger Petra, Stracke Jan Olaf, Hertenberger Hubert, Fingas Felix, Kling Lothar, Emrich Thomas, Drabner Georg, Seeber Stefan, Auer Johannes, Koch Stefan, Papadimitriou Apollon

Abstract excerpt

The neonatal Fc receptor (FcRn) is important for the metabolic fate of IgG antibodies in vivo. Analysis of the interaction between FcRn and IgG in vitro might provide insight into the structural and functional integrity of therapeutic IgG that may affect pharmacokinetics (PK) in vivo. We developed a standardized pH gradient FcRn affinity liquid chromatography method with conditions closely resembling the...

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Share to Antibodies, Monoclonal, Murine-Derived
Share to Chromatography, Affinity

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Histocompatibility Antigens Class I

Share to Hydrogen-Ion Concentration

Share to Immunoglobulin Fc Fragments

Share to Immunoglobulin G

Share to Receptors, Fc

Share to Surface Plasmon Resonance

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Identifiers

PMID: 23765230
DOI: 10.4161/mabs.24981
PMCID: PMC3906311

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