Article
Engineered human IgG antibodies with longer serum half-lives in primates.
The Journal of biological chemistry - 20 Feb 2004
Hinton Paul R, Johlfs Mary G, Xiong Joanna M, Hanestad Kelly, Ong Kelly C, Bullock Chuck, Keller Stephen, Tang Meina Tao, Tso J Yun, Vásquez Max, Tsurushita Naoya
Abstract excerpt
The neonatal Fc receptor (FcRn) plays an important role in regulating the serum half-lives of IgG antibodies. A correlation has been established between the pH-dependent binding affinity of IgG antibodies to FcRn and their serum half-lives in mice. In this study, molecular modeling was used to identify Fc positions near the FcRn binding site in a human IgG antibody that, when mutated, might alter the binding...
Topics
- Animals
- Antibodies
- Binding Sites
- Binding Sites, Antibody
- Binding, Competitive
- Cell Line
- Cloning, Molecular
- DNA, Complementary
- Dose-Response Relationship, Drug
- Half-Life
- Histocompatibility Antigens Class I
- Humans
- Hydrogen-Ion Concentration