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Characterization and preliminary mutation analysis of a thermostable alanine racemase from Thermoanaerobacter tengcongensis MB4.

Extremophiles : life under extreme conditions - 1 Jul 2013

Xue Zhangwei, Hu Yi, Xu Shujing, Ohnishi Kouhei, Ma Yanhe, Ju Jiansong, Zhao Baohua

Abstract excerpt

A thermostable alanine racemase from Thermoanaerobacter tengcongensis MB4 was successfully expressed in Escherichia coli and characterized. The full-length gene MBalr2 (1164 bp) encodes 388 amino acid residues including 6 out of 8 highly conserved amino acid residues at the entryway to the active site of alanine racemase. Recombinant MBAlr2 and three mutants (S171A, H359Y and double mutation S171A/H359Y) of...

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PMID: 23703245
DOI: 10.1007/s00792-013-0545-5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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