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Article

Identification and elucidation of in vivo function of two alanine racemases from Pseudomonas putida KT2440.

Environmental microbiology reports - 1 Oct 2017

Duque Estrella, Daddaoua Abdelali, Cordero Baldo F, De la Torre Jesús, Antonia Molina-Henares Maria, Ramos Juan-Luis

Abstract excerpt

The genome of Pseudomonas putida KT2440 contains two open reading frames (ORFs), PP_3722 and PP_5269, that encode proteins with a Pyridoxal phosphate binding motif and a high similarity to alanine racemases. Alanine racemases play a key role in the biosynthesis of D-alanine, a crucial amino acid in the peptidoglycan layer. For these ORFs, we generated single and double mutants and found that inactivation of...

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PMID: 28799718
DOI: 10.1111/1758-2229.12576

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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