Article
Detection of BRAF V600E mutation with thyroid tissue using pyrosequencing: comparison with PNA-clamping and real-time PCR.
American journal of clinical pathology - 1 Jun 2013
Kang Seong-Ho, Pyo Ju Yeon, Yang Seok-Woo, Hong Soon Won
Abstract excerpt
We used pyrosequencing, peptide nucleic acid (PNA)-clamping polymerase chain reaction (PCR), and real-time PCR to detect the BRAF V600E mutation and to investigate the prognostic effect of the BRAF V600E mutation in paraffin block specimens from 100 patients diagnosed with papillary thyroid carci...
Topics
- Adolescent
- Adult
- Aged
- Carcinoma
- Carcinoma, Papillary
- Humans
- Middle Aged
- Mutation
- Peptide Nucleic Acids
- Polymerase Chain Reaction
- Prognosis
- Proto-Oncogene Proteins B-raf
- Real-Time Polymerase Chain Reaction
- Sequence Analysis, DNA
- Thyroid Cancer, Papillary
- Thyroid Gland
- Thyroid Neoplasms