Article
DHPLC is a highly sensitive and rapid screening method to detect BRAF(V600E) mutation in papillary thyroid carcinoma.
Experimental and molecular pathology - 1 Feb 2013
Kim Tae Eun, Jung Eun Sun, Jung Chan Kwon, Bae Ja Seong, Kim Seung Nam, Kim Gyeong Suk, Lee Hyoung Nam, Kang Chang Suk, Choi Yeong Jin
Abstract excerpt
The BRAF(V600E) mutation has been reported to occur in 30% to 80% of papillary thyroid carcinomas (PTCs). Although direct sequencing is the method most commonly used to identify mutations, this technique is not sensitive enough to accurately detect low level mutation. To determine the optimal dia...
Topics
- Adult
- Aged
- Carcinoma
- Carcinoma, Papillary
- Chromatography, High Pressure Liquid
- Colorimetry
- DNA Mutational Analysis
- DNA, Neoplasm
- Female
- Humans
- Male
- Middle Aged
- Mutation
- Paraffin Embedding
- Proto-Oncogene Proteins B-raf