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Article

A combination of immunohistochemistry and molecular approaches improves highly sensitive detection of BRAF mutations in papillary thyroid cancer.

Endocrine - 1 Sept 2016

Martinuzzi Claudia, Pastorino Lorenza, Andreotti Virginia, Garuti Anna, Minuto Michele, Fiocca Roberto, Bianchi-Scarrà Giovanna, Ghiorzo Paola, Grillo Federica, Mastracci Luca

Abstract excerpt

The optimal method for BRAF mutation detection remains to be determined despite advances in molecular detection techniques. The aim of this study was to compare, against classical Sanger sequencing, the diagnostic performance of two of the most recently developed, highly sensitive methods: BRAF V600E immunohistochemistry (IHC) and peptide nucleic-acid (PNA)-clamp qPCR. BRAF exon 15 mutations were searched in...

Topics

Adenocarcinoma, Papillary
DNA Mutational Analysis
Female
Humans
Immunohistochemistry
Male
Middle Aged
Mutation
Proto-Oncogene Proteins B-raf
Real-Time Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Sensitivity and Specificity

Thyroid Neoplasms

Source

Identifiers

PMID: 26296380
DOI: 10.1007/s12020-015-0720-9

Genes

BRAF

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-26296380-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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