Article
Expression of a mutated bovine growth hormone gene suppresses growth of transgenic mice.
Proceedings of the National Academy of Sciences of the United States of America - 1 Jul 1990
Chen W Y, Wight D C, Wagner T E, Kopchick J J
Abstract excerpt
To determine the importance of the third alpha-helix in bovine growth hormone (bGH) relative to growth-related biological activities, the following experimental approach was used: (i) mutagenesis of helix III of bGH to generate an idealized amphiphilic helix; (ii) in vitro expression analyses of...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Cattle
- Cell Membrane
- Female
- Gene Expression
- Genes
- Growth Hormone
- Kinetics
- L Cells
- Liver
- Male
- Mice
- Mice, Transgenic
- Molecular Sequence Data
- Mutation
- Plasmids