Article
Mutations in the third alpha-helix of bovine growth hormone dramatically affect its intracellular distribution in vitro and growth enhancement in transgenic mice.
The Journal of biological chemistry - 5 Feb 1991
Chen W Y, Wight D C, Chen N Y, Coleman T A, Wagner T E, Kopchick J J
Abstract excerpt
To investigate the relationship between the secondary structure of the third alpha-helix (amino acids 109-126) of bovine growth hormone (bGH) and the biological activity of the molecule, proline or glycine residues have been used as substitutes for native amino acids at positions 114, 118, 121, and 126, respectively. Mutations at the positions 114, 118, and 121 resulted in a dramatic decrease in bGH secretion by...
Topics
- Animals
- Base Sequence
- Cattle
- Female
- Fluorescent Antibody Technique
- Gene Expression Regulation
- Growth Hormone
- L Cells
- Male
- Mice
- Mice, Transgenic