Article
Mutations in the paxillin-binding site of integrin-linked kinase (ILK) destabilize the pseudokinase domain and cause embryonic lethality in mice.
The Journal of biological chemistry - 28 Jun 2013
Moik Daniel, Böttcher Anika, Makhina Tatiana, Grashoff Carsten, Bulus Nada, Zent Roy, Fässler Reinhard
Abstract excerpt
Integrin-linked kinase (ILK) localizes to focal adhesions (FAs) where it regulates cell spreading, migration, and growth factor receptor signaling. Previous reports showed that overexpressed ILK in which Val(386) and Thr(387) were substituted with glycine residues (ILK-VT/GG) could neither interact with paxillin nor localize to FA in cells expressing endogenous wild-type ILK, implying that paxillin binding to ILK...
Topics
- Amino Acid Motifs
- Animals
- Binding Sites
- Cell Adhesion
- Cell Movement
- Embryo, Mammalian
- Flow Cytometry
- Focal Adhesions
- Gene Expression Regulation, Developmental
- Genes, Lethal
- Mice
- Microfilament Proteins
- Mutation