Article
The 5'- and 3'-untranslated regions of ornithine decarboxylase mRNA affect the translational efficiency.
The Journal of biological chemistry - 15 Jul 1990
Grens A, Scheffler I E
Abstract excerpt
We have determined the roles of the 5'- and 3'-untranslated regions (UTR) of ornithine decarboxylase (ODC) mRNA in the post-transcriptional regulation of this enzyme. A series of expression vectors were constructed in which portions of the ODC 5' and/or 3' UTRs were placed flanking a reporter gene coding sequence, either firefly luciferase or chloramphenicol acetyltransferase, so as to generate a hybrid...
Topics
- Animals
- Base Sequence
- Blotting, Northern
- Cell Line
- Chloramphenicol O-Acetyltransferase
- Genetic Vectors
- Luciferases
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Nucleic Acid Hybridization