Article
Single amino-acid replacement is responsible for the stabilization of ornithine decarboxylase in HMOA cells.
European journal of biochemistry - 15 Jun 1993
Miyazaki Y, Matsufuji S, Murakami Y, Hayashi S
Abstract excerpt
The half-life of ornithine decarboxylase (ODC) in HMOA cells, a variant cell line derived from hepatoma tissue culture (HTC) cells, is markedly increased compared with that in the parental cell line. In the present study, we examined which of the three relevant factors is responsible for the ODC...
Topics
- Animals
- Base Sequence
- Blotting, Northern
- Blotting, Southern
- CHO Cells
- Cell Line
- Cell-Free System
- Cricetinae
- Cysteine
- Enzyme Stability
- Genetic Vectors
- Mice
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Ornithine Decarboxylase
- RNA, Antisense
- RNA, Messenger