Article
An inducible transgene expression system for zebrafish and chick.
Development (Cambridge, England) - 1 May 2013
Gerety Sebastian S, Breau Marie A, Sasai Noriaki, Xu Qiling, Briscoe James, Wilkinson David G
Abstract excerpt
We have generated an inducible system to control the timing of transgene expression in zebrafish and chick. An estrogen receptor variant (ERT2) fused to the GAL4 transcriptional activator rapidly and robustly activates transcription within 3 hours of treatment with the drug 4-hydroxy-tamoxifen (4-OHT) in tissue culture and transgenic zebrafish. We have generated a broadly expressed inducible ERT2-GAL4 zebrafish...
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