Article
Mechanism of somatic hypermutation at the WA motif by human DNA polymerase η.
Proceedings of the National Academy of Sciences of the United States of America - 14 May 2013
Zhao Ye, Gregory Mark T, Biertümpfel Christian, Hua Yue-Jin, Hanaoka Fumio, Yang Wei
Abstract excerpt
Somatic hypermutation is programmed base substitutions in the variable regions of Ig genes for high-affinity antibody generation. Two motifs, RGYW and WA (R, purine; Y, pyrimidine; W, A or T), have been found to be somatic hypermutation hotspots. Overwhelming evidence suggests that DNA polymerase η (Pol η) is responsible for converting the WA motif to WG by misincorporating dGTP opposite the templating T. To...
Topics
- Adenosine Triphosphate
- Amino Acid Motifs
- Cations
- DNA-Directed DNA Polymerase
- Deoxyguanine Nucleotides
- Humans
- Immunoglobulins
- Kinetics
- Mutagenesis, Site-Directed
- Mutation
- Nucleic Acid Conformation
- Somatic Hypermutation, Immunoglobulin