Article
Effect of mutations in Candida antarctica B lipase.
Chemistry and physics of lipids - 1 Jun 1998
Patkar S, Vind J, Kelstrup E, Christensen M W, Svendsen A, Borch K, Kirk O
Abstract excerpt
Three variants of the Candida antarctica B lipase have been constructed and characterized. The variant containing the T103G mutation, which introduces the consensus sequence G-X-S-X-G found in most other known lipases, shows an increased thermostability but retains only half the specific activity...
Topics
- Candida
- Enzyme Stability
- Enzymes, Immobilized
- Fungal Proteins
- Hot Temperature
- Lipase
- Models, Molecular
- Mutagenesis, Site-Directed
- Mutation
- Oxidation-Reduction
- Stereoisomerism
- Substrate Specificity