Article
Preparation of a whole-cell biocatalyst of mutated Candida antarctica lipase B (mCALB) by a yeast molecular display system and its practical properties.
Applied microbiology and biotechnology - 1 Jun 2007
Kato Michiko, Fuchimoto Jun, Tanino Takanori, Kondo Akihiko, Fukuda Hideki, Ueda Mitsuyoshi
Abstract excerpt
To prepare a whole-cell biocatalyst of a stable lipase at a low price, mutated Candida antarctica lipase B (mCALB) constructed on the basis of the primary sequences of CALBs from C. antarctica CBS 6678 strain and from C. antarctica LF 058 strain was displayed on a yeast cell surface by alpha-agglutinin as the anchor protein for easy handling and stability of the enzyme. When mCALB was displayed on the yeast cell...
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