Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

KRAS and BRAF mutation analysis in colorectal adenocarcinoma specimens with a low percentage of tumor cells.

Molecular diagnosis & therapy - 1 Jun 2013

Lewandowska Marzena Anna, Jóźwicki Wojciech, Żurawski Bogdan

Abstract excerpt

BACKGROUND AND OBJECTIVE: The rapid development of molecular biology techniques allows for the introduction of real-time polymerase chain reaction (PCR) methods with a limit of mutation detection at 1% in a background of wild-type DNA. Analysis of KRAS mutations in codons 12, 13, and 61, together with analysis of BRAF mutations in codon 600, are predictive biomarkers for anti-epidermal growth factor receptor...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Biopsy
Share to Cell Count
Share to Fixatives

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Formaldehyde

Share to Limit of Detection

Share to Paraffin Embedding

Share to ras Proteins

Share to Real-Time Polymerase Chain Reaction

Source

Open in PubMed Open full text

Identifiers

PMID: 23606169
DOI: 10.1007/s40291-013-0025-8
PMCID: PMC3663254

Genes

KRAS
BRAF
EGFR

Variants

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

KRAS and BRAF mutation analysis in colorectal adenocarcinoma specimens with a low percentage of tumor cells.PMID 23606169Read ↗

[Molecular biological diagnostics of KRAS and BRAF mutations in patients with colorectal cancer - laboratory experience].Semantic proximity Read ↗

KRAS mutation testing in colorectal cancer as an example of the pathologist's role in personalized targeted therapy: a practical approach.Semantic proximity Read ↗

Extreme assay sensitivity in molecular diagnostics further unveils intratumour heterogeneity in metastatic colorectal cancer as well as artifactual low-frequency mutations in the KRAS gene.Semantic proximity Read ↗

KRAS mutations in Slovene patients with colorectal cancer: frequency, distribution and correlation with the response to treatment.Semantic proximity Read ↗

Highly sensitive KRAS mutation detection from formalin-fixed paraffin-embedded biopsies and circulating tumour cells using wild-type blocking polymerase chain reaction and Sanger sequencing.Semantic proximity Read ↗

Analysis of KRAS, NRAS, BRAF, and PIK3CA mutations could predict metastases in colorectal cancer: A preliminary study.Semantic proximity Read ↗

KRAS genotyping in rectal adenocarcinoma specimens with low tumor cellularity after neoadjuvant treatment.Semantic proximity Read ↗

KRAS mutation analysis on low percentage of colon cancer cells: the importance of quality assurance.Semantic proximity Read ↗

Low percentage of KRAS mutations revealed by locked nucleic acid polymerase chain reaction: implications for treatment of metastatic colorectal cancer.Semantic proximity Read ↗

The use of COLD-PCR and high-resolution melting analysis improves the limit of detection of KRAS and BRAF mutations in colorectal cancer.Semantic proximity Read ↗

KRAS genotyping as biomarker in colorectal cancer: a comparison of three commercial kits on histologic material.Semantic proximity Read ↗

Single-Tubed Wild-Type Blocking Quantitative PCR Detection Assay for the Sensitive Detection of Codon 12 and 13 KRAS Mutations.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.