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HicA Toxin-Based Counterselection Marker for Allelic Exchange Mutations in Fusobacterium nucleatum.

Applied and environmental microbiology - 26 Apr 2023

Gc Bibek, Zhou Peng, Wu Chenggang

Abstract excerpt

The study of fusobacterial virulence factors has dramatically benefited from the creation of various genetic tools for DNA manipulation, including galK-based counterselection for in-frame deletion mutagenesis in Fusobacterium nucleatum, which was recently developed. However, this method requires a host lacking the galK gene, which is an inherent limitation. To circumvent this limitation, we explored the...

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Identifiers

PMID: 37039662
DOI: 10.1128/aem.00091-23
PMCID: PMC10132090

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