Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Construction of non-polar mutants in Haemophilus influenzae using FLP recombinase technology.

BMC molecular biology - 11 Nov 2008

Tracy Erin, Ye Fang, Baker Beth D, Munson Robert S

Abstract excerpt

BACKGROUND: Nontypeable Haemophilus influenzae (NTHi) is a gram-negative bacterium that causes otitis media in children as well as other infections of the upper and lower respiratory tract in children and adults. We are employing genetic strategies to identify and characterize virulence determinants in NTHi. NTHi is naturally competent for transformation and thus construction of most mutants by common...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacteriophage lambda
Share to DNA Nucleotidyltransferases
Share to Fimbriae Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genetic Engineering

Share to Haemophilus influenzae

Share to Operon

Share to Transformation, Bacterial

Share to Viral Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 19014437
DOI: 10.1186/1471-2199-9-101
PMCID: PMC2625361

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Construction of non-polar mutants in Haemophilus influenzae using FLP recombinase technology.PMID 19014437Read ↗

Constructing unmarked gene deletions in Legionella pneumophila.Semantic proximity Read ↗

A new simple method for introducing an unmarked mutation into a large gene of non-competent Gram-negative bacteria by FLP/FRT recombination.Semantic proximity Read ↗

HicA Toxin-Based Counterselection Marker for Allelic Exchange Mutations in Fusobacterium nucleatum.Semantic proximity Read ↗

Site-directed mutagenesis and gene deletion using reverse genetics.Semantic proximity Read ↗

A HicA toxin-based counter-selection marker for allelic exchange mutations in Fusobacterium nucleatumSemantic proximity Read ↗

Donor plasmids for phenotypically neutral chromosomal gene insertions in Enterobacteriaceae.Semantic proximity Read ↗

In vitro and in vivo complementation of the Helicobacter pylori arginase mutant using an intergenic chromosomal site.Semantic proximity Read ↗

Use of the native flp gene to generate in-frame unmarked mutations in Streptomyces spp.Semantic proximity Read ↗

Broad-host-range cre-lox system for antibiotic marker recycling in gram-negative bacteria.Semantic proximity Read ↗

Versatile Vectors for Efficient Mutagenesis of Bradyrhizobium diazoefficiens and Other Alphaproteobacteria.Semantic proximity Read ↗

Use of lambda Red-mediated recombineering and Cre/lox for generation of markerless chromosomal deletions in avian pathogenic Escherichia coli.Semantic proximity Read ↗

Multiple genetic tools for editing the genome of Riemerella anatipestifer using a counterselectable marker.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.