Article

A HicA toxin-based counter-selection marker for allelic exchange mutations in Fusobacterium nucleatum

2023-01-21

Abstract excerpt

<h4>ABSTRACT</h4> The study of fusobacterial virulence factors has dramatically benefited from the creation of various genetic tools for DNA manipulation, including the galK-based counterselection for in-frame deletion mutagenesis in Fusobacterium nucleatum that was recently developed. However, this method requires a host lacking the galK gene, which is an inherent limitation. To circumvent this limitation, we...

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Literature Corpus work: 46017d80-3bc7-599a-b512-7ddc5a06588d
DOI: 10.1101/2023.01.20.524997

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