Article
Synergy between the NF-E1 erythroid-specific transcription factor and the CACCC factor in the erythroid-specific promoter of the human porphobilinogen deaminase gene.
Molecular and cellular biology - 1 Jul 1990
Frampton J, Walker M, Plumb M, Harrison P R
Abstract excerpt
A 114-base-pair promoter fragment of the human porphobilinogen deaminase gene functioned in an erythroid-specific manner in transient transfection experiments. Site-directed mutagenesis of the binding site for the erythroid-specific transcription factor (NF-E1) or an adjacent CACCC motif abolished the promoter activity. Increasing the spacing between these sites progressively reduced promoter activity, but there...
Topics
- Ammonia-Lyases
- Base Sequence
- Binding Sites
- Chromosome Deletion
- DNA-Binding Proteins
- Erythroid-Specific DNA-Binding Factors
- Gene Expression Regulation
- Genes
- Genetic Vectors
- Humans