Article
Cooperative and non-cooperative DNA binding modes of catabolite control protein CcpA from Bacillus megaterium result from sensing two different signals.
Journal of molecular biology - 7 Mar 1997
Gösseringer R, Küster E, Galinier A, Deutscher J, Hillen W
Abstract excerpt
Carbon catabolite repression (CCR) of several operons in Bacillus subtilis and Bacillus megaterium is mediated by the cis-acting cre sequence and trans-acting catabolite control protein (CcpA). We describe purification of CcpA from B. megaterium and its interaction with regulatory sequences from...
Topics
- Bacillus megaterium
- Bacterial Proteins
- DNA Footprinting
- DNA, Bacterial
- DNA, Recombinant
- DNA-Binding Proteins
- Electrophoresis, Polyacrylamide Gel
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Glucose
- Glucose-6-Phosphate
- Hydrogen-Ion Concentration
- Luciferases
- Mutation