Article
The Lactobacillus casei ptsHI47T mutation causes overexpression of a LevR-regulated but RpoN-independent operon encoding a mannose class phosphotransferase system.
Journal of bacteriology - 1 Jul 2004
Mazé Alain, Boël Grégory, Poncet Sandrine, Mijakovic Ivan, Le Breton Yoann, Benachour Abdellah, Monedero Vicente, Deutscher Josef, Hartke Axel
Abstract excerpt
A proteome analysis of Lactobacillus casei mutants that are affected in carbon catabolite repression revealed that a 15-kDa protein was strongly overproduced in a ptsHI47T mutant. This protein was identified as EIIA of a mannose class phosphotransferase system (PTS). A 7.1-kb DNA fragment containing the EIIA-encoding open reading frame and five other genes was sequenced. The first gene encodes a protein...
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