Article
Phenotypic knockout of the high-affinity human interleukin 2 receptor by intracellular single-chain antibodies against the alpha subunit of the receptor.
Proceedings of the National Academy of Sciences of the United States of America - 11 Apr 1995
Richardson J H, Sodroski J G, Waldmann T A, Marasco W A
Abstract excerpt
The experimental manipulation of peptide growth hormones and their cellular receptors is central to understanding the pathways governing cellular signaling and growth control. Previous work has shown that intracellular antibodies targeted to the endoplasmic reticulum (ER) can be used to capture specific proteins as they enter the ER, preventing their transport to the cell surface. Here we have used this...
Topics
- Amino Acid Sequence
- Antibodies
- Base Sequence
- Cell Compartmentation
- Endoplasmic Reticulum
- Genetic Therapy
- Humans
- Immunoglobulin Fragments
- Immunoglobulin Variable Region
- Leukemia, T-Cell