Article
Reference-free SNP discovery for the Eurasian beaver from restriction site-associated DNA paired-end data.
Molecular ecology - 1 Jun 2013
Senn Helen, Ogden Rob, Cezard Timothee, Gharbi Karim, Iqbal Zamin, Johnson Eric, Kamps-Hughes Nick, Rosell Frank, McEwing Ross
Abstract excerpt
In this study, we used restriction site-associated DNA (RAD) sequencing to discover SNP markers suitable for population genetic and parentage analysis with the aim of using them for monitoring the reintroduction of the Eurasian beaver (Castor fibre) to Scotland. In the absence of a reference genome for beaver, we built contigs and discovered SNPs within them using paired-end RAD data, so as to have sufficient...
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