Article
ddRAD sequencing-based identification of inter-genepool SNPs and association analysis in Brassica juncea.
BMC plant biology - 30 Dec 2019
Sudan Jebi, Singh Ravinder, Sharma Susheel, Salgotra Romesh K, Sharma Varun, Singh Gurvinder, Sharma Indu, Sharma Swarkar, Gupta Surinder K, Zargar Sajad Majeed
Abstract excerpt
BACKGROUND: Narrow genetic base, complex allo-tetraploid genome and presence of repetitive elements have led the discovery of single nucleotide polymorphisms (SNPs) in Brassica juncea (AABB; 2n = 4x = 36) at a slower pace. Double digest RAD (ddRAD) - a genome complexity reduction technique followed by NGS was used to generate a total of 23 million paired-end reads from three genotypes each of Indian (Pusa Tarak,...
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