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Genomic exploration and molecular marker development in a large and complex conifer genome using RADseq and mRNAseq.

Molecular ecology resources - 1 May 2015

Karam M-J, Lefèvre F, Dagher-Kharrat M Bou, Pinosio S, Vendramin G G

Abstract excerpt

We combined restriction site associated DNA sequencing (RADseq) using a hypomethylation-sensitive enzyme and messenger RNA sequencing (mRNAseq) to develop molecular markers for the 16 gigabase genome of Cedrus atlantica, a conifer tree species. With each method, Illumina(®) reads from one individual were used to generate de novo assemblies. SNPs from the RADseq data set were detected in a panel of one single...

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PMID: 25224750
DOI: 10.1111/1755-0998.12329

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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