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Article

A reference-free approach to analyse RADseq data using standard next generation sequencing toolkits.

Molecular ecology resources - 1 May 2021

Heller Rasmus, Nursyifa Casia, Garcia-Erill Genís, Salmona Jordi, Chikhi Lounes, Meisner Jonas, Korneliussen Thorfinn Sand, Albrechtsen Anders

Abstract excerpt

Genotyping-by-sequencing methods such as RADseq are popular for generating genomic and population-scale data sets from a diverse range of organisms. These often lack a usable reference genome, restricting users to RADseq specific software for processing. However, these come with limitations compared to generic next generation sequencing (NGS) toolkits. Here, we describe and test a simple pipeline for...

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PMID: 33434329
DOI: 10.1111/1755-0998.13324

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