Glow-type conversion and characterization of a minimal luciferase via mutational analyses.

doi:10.1111/febs.16937

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Article

Glow-type conversion and characterization of a minimal luciferase via mutational analyses.

The FEBS journal - 1 Dec 2023

Ohmuro-Matsuyama Yuki, Matsui Hayato, Kanai Masaki, Furuta Tadaomi

Abstract excerpt

Luciferases are widely used as reporter proteins in various fields. Recently, we developed a minimal bright luciferase, picALuc, via partial deletion of the artificial luciferase (ALuc) derived from copepods luciferases. However, the structures of copepod luciferases in the substrate-bound state remain unknown. Moreover, as suggested by structural modeling, picALuc has a larger active site cavity, unlike that in...

Topics

Amino Acid Sequence
Luciferases
Mutation
Catalytic Domain
Catalysis
Luminescent Measurements

Source

Open in PubMed

Identifiers

PMID: 37622174
DOI: 10.1111/febs.16937

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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