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Article

Advanced Bioluminescence Reporter with Engineered Gaussia Luciferase via Sequence-Guided Mutagenesis.

Biosensors - 1 Nov 2024

Gedi Vinayakumar, Kim Eun Hye, Oh Bohyun, Kim Young-Pil

Abstract excerpt

Gaussia luciferase (GLuc) is the preeminent secreted luciferase widely used in cell-based reporter assays. By employing sequence-guided mutagenesis informed by alignments of diverse copepod luciferase sequences, we identified key amino acids that significantly enhance bioluminescence (BL) intensity. Among the mutated proteins expressed in bacteria, five individual mutations (M60L, K88Q, F89Y, I90L, or S103T)...

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Identifiers

PMID: 39589987
DOI: 10.3390/bios14110528
PMCID: PMC11591556

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