Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Superluminescent variants of marine luciferases for bioassays.

Analytical chemistry - 15 Nov 2011

Kim Sung Bae, Suzuki Hideyuki, Sato Moritoshi, Tao Hiroaki

Abstract excerpt

In this study, a rational synthesis of superluminescent variants from marine luciferases with prolonged bioluminescence has been demonstrated. A putative active site of a model marine luciferase, Gaussia princeps Luciferase (GLuc), was assigned and modified by a site-directed mutagenesis. The potent variants were found to generate up to 10 times stronger bioluminescence, emitting red shifts of up to 33 nm with...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Biological Assay
Share to Chlorocebus aethiops
Share to COS Cells

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Crustacea

Share to Luciferases

Share to Luminescence

Share to Luminescent Measurements

Share to Mutagenesis

Source

Identifiers

PMID: 21951281
DOI: 10.1021/ac2021882

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Superluminescent variants of marine luciferases for bioassays.PMID 21951281Read ↗

Advanced Bioluminescence Reporter with Engineered Gaussia Luciferase via Sequence-Guided Mutagenesis.Semantic proximity Read ↗

Multiply mutated Gaussia luciferases provide prolonged and intense bioluminescence.Semantic proximity Read ↗

Directed molecular evolution reveals Gaussia luciferase variants with enhanced light output stability.Semantic proximity Read ↗

A Very Bright Far-Red Bioluminescence Emitting Combination Based on Engineered Railroad Worm Luciferase and 6'-Amino-Analogs for Bioimaging Purposes.Semantic proximity Read ↗

LuxSit Pro and sPro: Next-Generation Designed Luciferases for Bioluminescent Reporting and Complementation-Based DetectionSemantic proximity Read ↗

Aminoluciferins extend firefly luciferase bioluminescence into the near-infrared and can be preferred substrates over D-luciferin.Semantic proximity Read ↗

Glow-type conversion and characterization of a minimal luciferase via mutational analyses.Semantic proximity Read ↗

Bioluminescence assays: multicolor luciferase assay, secreted luciferase assay and imaging luciferase assaySemantic proximity Read ↗

Elucidating Catalytic Bioluminescence of Mammalian Gaussia Luciferase through Mutant and Ancestral Analysis.Semantic proximity Read ↗

Continuous Hypermutation and Evolution of Luciferase Variants.Semantic proximity Read ↗

Theoretical Development of Near-Infrared Bioluminescent Systems.Semantic proximity Read ↗

Thermostabilization of firefly luciferase by in vivo directed evolution.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.