Article
DNA typing for class II HLA antigens with allele-specific or group-specific amplification. II. Typing for alleles of the DRw52-associated group.
Human immunology - 1 May 1990
Fernandez-Vina M, Shumway W, Stastny P
Abstract excerpt
Codons 9-12 of the first domain of DRB1, which encode the amino acid sequence EYST (in single-letter code), served as the basis for the construction of a polymerase chain reaction primer specific for DRB1 of the whole DRw52 group. Using this primer for the 5' end and a primer for a conserved region at the 3' end, we could amplify selectively the DRB1 genes of DRw17-, w18-, w11-, w13-, w14, and w8-positive...
Topics
- Alleles
- Base Sequence
- Cell Line
- Codon
- DNA
- DNA Probes, HLA
- Electrophoresis, Polyacrylamide Gel
- Gene Amplification
- HLA-DR Antigens
- HLA-DR Serological Subtypes
- Histocompatibility Testing