Article
An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system.
Proceedings of the National Academy of Sciences of the United States of America - 23 Jul 2002
Kiga Daisuke, Sakamoto Kensaku, Kodama Koichiro, Kigawa Takanori, Matsuda Takayoshi, Yabuki Takashi, Shirouzu Mikako, Harada Yoko, Nakayama Hiroshi, Takio Koji, Hasegawa Yoshinori, Endo Yaeta, Hirao Ichiro, Yokoyama Shigeyuki
Abstract excerpt
Tyrosyl-tRNA synthetase (TyrRS) from Escherichia coli was engineered to preferentially recognize 3-iodo-L-tyrosine rather than L-tyrosine for the site-specific incorporation of 3-iodo-L-tyrosine into proteins in eukaryotic translation systems. The wild-type TyrRS does not recognize 3-iodo-L-tyros...
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