Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

An unnatural base pair for incorporating amino acid analogs into proteins.

Nature biotechnology - 1 Feb 2002

Hirao Ichiro, Ohtsuki Takashi, Fujiwara Tsuyoshi, Mitsui Tsuneo, Yokogawa Tomoko, Okuni Taeko, Nakayama Hiroshi, Takio Koji, Yabuki Takashi, Kigawa Takanori, Kodama Koichiro, Yokogawa Takashi, Nishikawa Kazuya, Yokoyama Shigeyuki

Abstract excerpt

An unnatural base pair of 2-amino-6-(2-thienyl)purine (denoted by s) and pyridin-2-one (denoted by y) was developed to expand the genetic code. The ribonucleoside triphosphate of y was site-specifically incorporated into RNA, opposite s in a template, by T7 RNA polymerase. This transcription was coupled with translation in an Escherichia coli cell-free system. The yAG codon in the transcribed ras mRNA was...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Tyrosine
Share to Amino Acids

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Cell-Free System

Share to DNA-Directed RNA Polymerases

Share to Gas Chromatography-Mass Spectrometry

Share to Models, Chemical

Share to Protein Engineering

Share to RNA, Transfer

Source

Identifiers

PMID: 11821864
DOI: 10.1038/nbt0202-177

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

An unnatural base pair for incorporating amino acid analogs into proteins.PMID 11821864Read ↗

A chemical toolkit for proteins--an expanded genetic code.Semantic proximity Read ↗

Five-base codons for incorporation of nonnatural amino acids into proteins.Semantic proximity Read ↗

Functional replacement of the endogenous tyrosyl-tRNA synthetase-tRNATyr pair by the archaeal tyrosine pair in Escherichia coli for genetic code expansion.Semantic proximity Read ↗

Genetic code expansion in mammalian cellsSemantic proximity Read ↗

Incorporation of 3-azidotyrosine into proteins through engineering yeast tyrosyl-tRNA synthetase and its application to site-selective protein modification.Semantic proximity Read ↗

Site-specific incorporation of a fluorescent terphenyl unnatural amino acid.Semantic proximity Read ↗

An expanded eukaryotic genetic code.Semantic proximity Read ↗

A simple system for expression of proteins containing 3-azidotyrosine at a pre-determined site in Escherichia coli.Semantic proximity Read ↗

Phosphoserine aminoacylation of tRNA bearing an unnatural base anticodon.Semantic proximity Read ↗

Expanding the genetic code.Semantic proximity Read ↗

An engineered Escherichia coli tyrosyl-tRNA synthetase for site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat germ cell-free system.Semantic proximity Read ↗

Rational design of aminoacyl-tRNA synthetase specific for p-acetyl-L-phenylalanine.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.