Article
The model of amyloid aggregation of Escherichia coli RNA polymerase σ70 subunit based on AFM data and in vitro assays.
Cell biochemistry and biophysics - 1 Jul 2013
Koroleva Olga N, Dubrovin Evgeniy V, Khodak Yu A, Kuzmina Natalia V, Yaminsky Igor V, Drutsa Valeriy L
Abstract excerpt
To propose a model for recently described amyloid aggregation of E.coli RNA polymerase σ(70) subunit, we have investigated the role of its N-terminal region. For this purpose, three mutant variants of protein with deletions Δ1-73, Δ1-100 and Δ74-100 were constructed and studied in a series of in vitro assays and using atomic force microscopy (AFM). Specifically, all RNA polymerase holoenzymes, reconstituted with...
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