Article
Escherichia coli processivity clamp β from DNA polymerase III is dynamic in solution.
Biochemistry - 5 Jul 2011
Fang Jing, Engen John R, Beuning Penny J
Abstract excerpt
Escherichia coli DNA polymerase III is a highly processive replicase because of the presence of the β clamp protein that tethers DNA polymerases to DNA. The β clamp is a head-to-tail ring-shaped homodimer, in which each protomer contains three structurally similar domains. Although multiple studies have probed the functions of the β clamp, a detailed understanding of the conformational dynamics of the β clamp in...
Topics
- DNA Polymerase III
- Escherichia coli
- Mass Spectrometry
- Models, Molecular
- Mutagenesis, Site-Directed
- Mutation
- Protein Multimerization
- Protein Structure, Quaternary
- Protein Unfolding
- Solutions