Article
RNA editing of apolipoprotein B mRNA. Sequence specificity determined by in vitro coupled transcription editing.
The Journal of biological chemistry - 25 Apr 1990
Chen S H, Li X X, Liao W S, Wu J H, Chan L
Abstract excerpt
Apolipoprotein (apo) B-48 mRNA is produced by in vivo RNA editing which involves a C----U conversion of the first base of the codon CAA for Gln-2153, changing it to UAA, an in-frame stop codon. We have reproduced the editing reaction in vitro using nuclear extracts. Efficient RNA editing was demonstrated by using apoB mRNA segments as substrate or in a coupled transcription-editing reaction using apoB minigenes...
Topics
- Animals
- Apolipoproteins B
- Base Sequence
- Cell Nucleus
- Codon
- Genes
- Genetic Variation
- Humans
- Liver
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Oligonucleotide Probes