Elimination of apolipoprotein B48 formation in rat hepatoma cell lines transfected with mutant human apolipoprotein B cDNA constructs.

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Article

Elimination of apolipoprotein B48 formation in rat hepatoma cell lines transfected with mutant human apolipoprotein B cDNA constructs.

The Journal of biological chemistry - 15 Jan 1992

Yao Z M, Blackhart B D, Johnson D F, Taylor S M, Haubold K W, McCarthy B J

Abstract excerpt

Rat hepatoma McA-RH7777 cell lines transfected with full-length human apolipoprotein (apo) B constructs produce mostly human apoB48 and only small amounts of apoB100, as a result of mRNA editing at codon 2153 (C to U conversion at nucleotide 6666). To abolish the formation of apoB48 and increase the yield of apoB100 and other forms of apoB longer than apoB48, site-specific mutations were introduced at or near the...

Topics

Amino Acid Sequence
Animals
Apolipoprotein B-48
Apolipoproteins B
Base Sequence
Blotting, Western
DNA
Electrophoresis, Polyacrylamide Gel
Gene Expression
Humans
Hydrolysis

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Liver Neoplasms, Experimental

Molecular Sequence Data

Mutation

Plasmids

RNA, Messenger

Rats

Transfection

Source

Open in PubMed

Identifiers

PMID: 1730641

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1730641-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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